Figure 3.

Measurement for collagen metabolism of D-gal induced aging skin after each treatment. Skin wound was processed and analyzed for expression of collagen type I (a), collagen type III (b), MMP-1 (c) and its inhibitor, and TIMP-1 (d) by quantitative RT-PCR. Expression levels for each targeting gene were normalized to endogenous reference gene and reported as relative values (ΔΔCt) to those obtained from aging control group. ∗ and † indicate a significant difference (P < 0.05) when compared to negative control (group 1) and sham control (group 8), respectively.