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. Author manuscript; available in PMC: 2014 Aug 6.
Published in final edited form as: Am J Respir Cell Mol Biol. 1998 Jun;18(6):800–812. doi: 10.1165/ajrcmb.18.6.3063

Figure 3.

Figure 3

Effect of hydroxyurea treatment on lymphocyte accumulation in the lungs. SRBC-primed C57BL/6 mice were challenged intratracheally with SRBC to induce a pulmonary immune response. Two days later, hydroxyurea (1 mg/g body weight) or saline was administered IP for 24 hours (three separate injections at eight hour intervals). Mice were killed humanely 20 minutes after the last injection and cells were harvested from the lungs by BAL and from the bone marrow. A, B. Representative histograms of bone marrow from individual mice treated with saline (A) or hydroxyurea (B), showing effective systemic ablation of dividing cells in hydroxyurea-treated mice. C. Total and differential cell recovery in BAL. Control (saline injected) mice are represented by dark cross-hatching and hydroxyurea-treated mice by light cross-hatching. Data are mean ± SEM of ten mice per group assayed in two separate experiments. There are no significant differences between saline and hydroxyurea-treated groups, p >0.05, unpaired t test.