XNC10-deficient 15/0 tumor cells are rejected by syngeneic LG-15 larval hosts. (A) LG-15 tadpoles were intraperitoneally injected with 1×105 15/0 WT, shScramble, shXNC10 clone #4 or #7 tumor cells and peritoneal exudates were collected and total cells from individual animals were counted 7 days posttransplantation. (B and C), Total peritoneal cells retrieved from 15/0 WT, shScramble, shXNC10 clone #4 or #7 tumor-transplanted recipients were combined into respective groups and stained with anti-CTX-APC. (B) Representative flow cytometry scatter plots of anti-CTX-APC of 15/0 WT and shXNC10#7 transplanted animals. (C) Quantification of CTX+ tumor cells in WT, shScramble, shXNC10 clone #4 or #7 transplanted animals. n = 4 independent experiments, with 3–5 animals per experiment.