Fig. 5.

Differential immune cell recruitment within tadpole peritoneum following WT and XNC10-deficient tumor cell transplantation. Tadpoles were IP injected with 1×10⁵ WT or shXNC10 #7 15/0 tumor cells, whereas control animals were injected with an equal volume of amphibian phosphate-buffered saline. Peritoneal cells were collected 7 days posttransplantation and counted for each individual. Cells for each group were then pooled, immune-stained for XNC10-T-APC and CD8-FITC and analyzed by flow cytometry. Dead cells were excluded using propidium iodide. (A) Representative flow plots showing XNC10-T-APC- and CD8-FITC-stained peritoneal exudates from amphibian phosphate-buffered saline, WT and XNC10-deficient transplanted animals. (B) Percent of iT cells calculated for each individual. Total number of C, iT; D, CD8 and E, Class II per 1000 tumor cells was determined for both WT and XNC10-deficient peritoneal exudates. Three independent experiments with give to eight animals per group were performed. P-values indicated within each histogram were determined using One way ANOVA (B) or Student’s t-test (C–E).