Fig. 4.

Apc^mNLS/mNLS mice have goblet cell defects. (A–D) Alcian Blue-stained sections of colons in DSS-treated Apc^+/+ (A and B) and Apc^mNLS/mNLS mice (C and D). Left panels show the whole Swiss roll. The squared areas in the left panels are magnified in right panels. (E and F) Relative expression of Muc2 mRNA in DSS-treated (E) and untreated (F) Apc^+/+ and Apc^{mNLS/ mNLS} mice (*P < 0.05, using Mann–Whitney test). (G) Goblet cells represented as percentage of total cells per crypt in jejunum from untreated Apc^+/+ and Apc^mNLS/mNLS mice (*P < 0.05, using mixed statistical model as described in Materials and methods). Error bars throughout represent standard error of the mean.