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Infusion with 250 mM D-glucose leads to a 2.9-fold up-regulation in jejunal SGLT1 protein expression (A, *P < 0.05 compared with water infusion), but no change in SGLT1 mRNA. Expression is shown relative to water infusion. These effects are specific to SGLT1, with no effect when looking at GLUT2, either at transcriptional level or on Western blotting (B). Similarly, no effect is seen when infusing 250 mM D-mannitol compared with water: protein densitometry results are shown in (C).
