FIGURE 4.

A–C, are schematic representations of isolated regional infusions. In (A), a clip placed at the ligament of Trietz, and a drainage catheter in situ, allow infusion of just the stomach and duodenum (labeled D in [Fig. D, E]). In (B), a clip is placed at both ligament of Trietz and at 15 cm distal to this, infusing only proximal jejunum (PJ). Lastly, in (C), infusion is restricted to distal jejunum and ileum (DJ). D, shows densitometry results for Western blots of protein extracts taken from proximal jejunum in each case, compared with whole bowel water infusion. Whole bowel glucose infusion is also shown for comparison. E, shows a representative SGLT1 immunoblot from proximal jejunal protein extracts obtained after regional infusion. WI, Whole intestine; D, Duodenum; PJ, proximal jejunum; DJ, Distal jejunum. *P < 0.05 compared with water; **P < 0.01 compared with water.