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. Author manuscript; available in PMC: 2015 Apr 10.
Published in final edited form as: Cell. 2014 Apr 10;157(2):357–368. doi: 10.1016/j.cell.2014.02.053

Figure 3. Single-cell concurrent measurement of Ca2+ flux and in situ TCR–pMHC bond kinetics.

Figure 3

(A–B) Representative wide-field pseudo-colored images of two types of intracellular Ca2+ signals. (C–D) Representative time courses of relative fura-2 ratio of type α (C, magenta curve, left ordinate) or type β (D, green curve, left ordinate) intracellular Ca2+ signal synchronized with concurrent measurement of rupture (red open circle) and lifetime (blue solid triangle) events and the cumulative lifetime (blue dashed curve, right ordinate). Note that there is no rupture events for Panel (C). A dashed-dot horizontal line indicates a 10-s threshold of cumulative lifetime. See also Figure S3.