Figure 2. The implications of harboring Spiroplasma under host nutritional depravation.
(A) Survival of virgin flies on a nutritionally poor diet. Flies-harboring Spiroplasma (Sp (+)) do not have significantly different mortality from flies that do not harbor Spiroplasma (Sp (−)). NS (p=0.9378). N = 50 flies per condition. Shown is one representative experiment out of three independent experiments. (B) The number of eggs laid by virgin flies-harboring Spiroplasma (Sp (+)) relative to control flies that do not harbor Spiroplasma (Sp (−)), in total over 14 days (left panel) and in 2-day period over 14 days (right panel). Overall, there is no significant difference in the number of eggs laid between Spiroplasma-infected and uninfected virgin flies under nutrient deprivation. NS (p=0.77). Shown is the mean ± SEM of data pooled from four independent experiments, N = 20 flies per experiment. (C) Quantification of Spiroplasma titers by qPCR reveals that virgin female flies maintained on the same nutritionally poor diet as in panel A have significantly lower Spiroplasma titers after 8 and 12 days than flies maintained on a rich diet. *p=0.015 and ***p=0.0002, respectively. Values for each time-point have at least three samples (five flies pooled per sample). Shown is the mean ± SEM of one representative experiment out of the three independent experiments that were conducted.
Figure 2—figure supplement 1. The effects of nutrient deprivation on survival and egg production of Spiroplasma-infected mated females.
Figure 2—figure supplement 2. Spiroplasma titers in fly hemolymph under nutrient deprivation.
