Figure 4. Spiroplasma proliferation is influenced by the nutrient composition of the diet.
Quantification of Spiroplasma titers by qPCR reveals that complementing nutrient poor media with inactivated yeast results in a significant increase in Spiroplasma titers after 12 days. ***p=0.0003. In contrast, complementing nutrient poor media with sucrose does not significantly increase Spiroplasma titers. NS (p=0.5). Values are the mean ± SEM of at least four samples (five flies per sample). Shown is one representative experiment out of the three independent experiments that were conducted.