Table 1.
Characterization and quantification of hemolymph of DAG species
| Component name | Sp − (µg/ml) | Sp + (µg/ml) | % Of total mass decline |
|---|---|---|---|
| C28:0 DAG | 13.4 | 12.3 | 7 |
| C28:1 DAG | 0.8 | 0.6 | 1.2 |
| C30:2 DAG | 0.7 | 0.5 | 1.2 |
| C30:0 DAG | 0.1 | 0.2 | −1.0 |
| C32:3 DAG | 1.2 | 1.1 | 0.7 |
| C32:2 DAG | 23.1 | 21.8 | 8.2 |
| C32:1 DAG | 19.8 | 15.1 | 30.1 |
| C34:2 DAG | 11.5 | 10.8 | 4.9 |
| C34:1 DAG | 9.7 | 6.9 | 19.3 |
| C34:0 DAG | 1.4 | 0.5 | 5.6 |
| C36:4 DAG | 0.8 | 0.5 | 1.8 |
| C36:3 DAG | 2.9 | 2.2 | 5.3 |
| C36:2 DAG | 4.2 | 2.9 | 9.3 |
| C36:1 DAG | 0.8 | 0.9 | −0.3 |
| C36:0 DAG | 1.7 | 0.7 | 6.9 |
| 92.1 | 77 |
Quantification of the absolute concentration of individual DAG species in the hemolymph of Spiroplasma-uninfected (Sp (−)) and Spiroplasma-infected (Sp (+)) mated flies by LC-MS/MS. The % of total mass decline reflects the percentage of the total decline between Spiroplasma-uninfected and infected samples (a total of 15.1 μg/ml or 16.3%) that can be attributed to each DAG species. It is notable that C32:1 and C34:1 DAG species decline to a greater extent than other common DAG species such as C28:0 and C32:2. This suggests that Spiroplasma is preferentially incorporating DAGs that have one saturated and one mono-unsaturated acyl chain. Notably, C34:1 DAGs are likely to be made up of oleic (C18:1) and palmitic (C16:0) acids, which have exactly the same acyl chains that were identified in Spiroplasma-generated cardiolipins (Figure 6B).