. 2014 Aug 6;5:404. doi: 10.3389/fmicb.2014.00404

Table 3.

Consumption of nitrite and oxygen and production of nitrous oxide by wild-type and norZ_ch mutant cultures of S. wittichii RW1 initiated at atmospheric oxygen headspace and with 0.3 or 1 mM NaNO₂.

Time (h)	NO⁻₂ remaining (mM)				%O₂ remaining in the headspace				N₂O produced (nmolOD⁻¹)
	WT		*norZ_ch*		WT		*norZ_ch*		WT		*norZ_ch*
	0.3 mM	1.0 mM	0.3 mM	1.0 mM	0.3 mM	1.0 mM	0.3 mM	1.0 mM	0.3 mM	1.0 mM	0.3 mM	1.0 mM
48	0.24^a ^b	0.78^a	0.27^a ^b	0.79^a	7.24^a ^b	7.62^a	7.82^b	7.55	2.66^a ^b	3.19^a ^b	1.76^a ^b	2.40^a ^b
72	0.01^a	0.24^a ^b	0.04^a	0.33^a ^b	4.50^b	4.65	4.71^b	4.63	10.30^a ^b	34.68^a ^b	8.80^a ^b	24.99^a ^b
96	0.00	0.01	0.00	0.02	4.19^b	4.12	4.42^b	4.45	17.69^a ^b	63.62^a ^b	14.79^a ^b	49.31^a ^b
120	0.00	0.00	0.00	0.00	4.31	3.89	4.48	4.33	19.28^a ^b	73.06^a ^b	16.83^a ^b	59.91^a ^b

Values represent averages from 5 experiments initiated on different cultures and on different days. N₂O amounts were normalized to OD of each culture at each time point. Statistically significant differences between treatments were determined by Student's t-test at p < 0.05 and are designated as follows:

Significant difference between 0.3 and 1 mM NaNO₂ treatment groups of wildtype (WT) or norZ_ch mutant cultures of S. wittichii RW1 at each time point.

Significant difference between WT and norZ_ch mutant S. wittichii RW1 cultures incubated with 0.3 mM NaNO₂ or 1 mM NaNO₂.