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TOYOPEARL CM-650M column chromatography for A. kurodai cellulase. Proteins precipitated between 40 and 60% saturation of ammonium sulfate from the crude enzyme was applied to a TOYOPEARL CM-650M column (1.5 × 20 cm) and eluted with a linear gradient of 0–0.3 M NaCl in 10 mM sodium phosphate buffer (pH 7.0) at a flow rate of 15 mL/h. Each fraction contains 7.0 mL. SDS-PAGE of the peak fractions are shown in the inset.
