Table 2.
N-terminal and internal amino-acid sequences of AkEG21.
| Peptidesa | Amino-acid sequences | Similarity (%) to other molluscan GHF45 enzymesb |
|---|---|---|
| AkEG21 | EQKCQPNSHGVRVYQGKKCA- | A. crossean (60.0%, 17–36) |
| H. discus discus (55.0%, 18–36) | ||
| L-1 | VNDHGYEAHFDLQNNK | A. crossean (68.8%, 143–158) |
| H. discus discus (68.8%, 141–156) | ||
| L-2 | LTPTGGFVPGNGK | A. crossean (84.6%, 95–107) |
| H. discus discus (61.5%, 92–104) | ||
| T-1 | CQPNSHGVR | A. crossean (77.8%, 20–28) |
| H. discus discus (44.4%, 21–28) | ||
| T-2 | VWCGQSGKPGTNK | A. crossean (61.5%, 130–142) |
| H. discus discus (46.2%, 128–140) | ||
| T-3 | YNDGHR | A. crossean (66.7%, 41–46) |
| H. discus discus (83.3%, 41–46) |
a
L-1 and L-2, Lysylendopeptidyl fragments; T-1–T-3, tryptic fragments.
b
Residue numbers for corresponding regions in the sequences of A. crossean and H. discus discus (see Figure 6) are also shown in the parentheses.