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. Author manuscript; available in PMC: 2015 Jul 15.
Published in final edited form as: Cancer Res. 2014 May 8;74(14):3716–3726. doi: 10.1158/0008-5472.CAN-13-3116

Figure 1. Exogenous netrin-1 induces MB cell invasiveness.

Figure 1

A, Four MB cell lines and brain capillary EC were cultured in Matrigel-coated Transwell chambers with indicated netrin-1 concentrations at 16 hours. Invading cells on the membrane were counted in four different fields. B, VEGF-A was analyzed on the same MB cell lines. C, For proliferation, MB cells (2.5 × 104) were cultured in serum reduced medium (0.5% FBS) in the absence or presence of netrin-1. Cell number was counted at 24 and 48 hours. Data represent the mean ± SD (n=3), * p < 0.05.