Table I.
Expression of chemokines and their related molecules in mouse retinas after light exposure
| genes | Abca4-/- Rdh8-/- mice1, 2 | WT | |||
|---|---|---|---|---|---|
| (24 h) | (7 days) | (24 h) | (7 days) | ||
| CC chemokine | Ccl2 | 30.52 | 1.13 | -1.40 | -2.93 |
| Ccl3 | 84.59 | 27.14 | 1.83 | -2.09 | |
| Ccl4 | 34.29 | 6.52 | 1.41 | -4.26 | |
| Ccl5 | 4.45 | 3.61 | 4.68 | 1.80 | |
| Ccl7 | 9.56 | -2.94 | -2.04 | -3.12 | |
| Ccl8 | -2.60 | -3.39 | -3.00 | -10.26 | |
| Ccl11 | -4.13 | -9.28 | -3.21 | -6.49 | |
| Ccl12 | 13.57 | 1.92 | 1.73 | -3.28 | |
| CC chemokine receptor | Ccr2 | 5.95 | 7.84 | -9.32 | -25.05 |
| Ccr3 | 29.04 | 31.76 | -1.21 | -1.19 | |
| Ccr6 | -3.01 | -3.39 | -1.23 | -2.76 | |
| Ccr7 | 1.62 | 3.88 | -1.51 | -1.44 | |
| Ccr10 | 2.46 | 3.51 | 7.62 | 3.43 | |
| CXC chemokine | Cxcl1 | 4.68 | -1.36 | 1.62 | -2.93 |
| Pf4 | -5.16 | -1.68 | -3.55 | -3.57 | |
| Cxcl5 | 7.26 | -2.01 | 8.96 | 8.53 | |
| Cxcl10 | 21.10 | 1.73 | 13.15 | -2.00 | |
| CXC chemokine receptor | Cxcr5 | 3.32 | 5.09 | 1.48 | 1.10 |
| Interleukin | Il1b | 6.44 | 1.47 | -1.25 | 1.22 |
| Il1f6 | -3.45 | -1.82 | -4.06 | -2.77 | |
| Il1f8 | -15.87 | -32.42 | 2.97 | 1.22 | |
| Il4 | 8.88 | 3.07 | 4.61 | 2.97 | |
| Il16 | 3.01 | 1.16 | -1.74 | -2.93 | |
| Il20 | -3.76 | -3.05 | -1.45 | -3.79 | |
| Interleukin receptor | Il1r1 | 1.69 | 1.46 | 1.64 | 1.61 |
| Il1r2 | -5.14 | -6.36 | -2.67 | -1.77 | |
| Il5ra | 4.35 | 6.16 | 2.91 | 2.52 | |
| Il6ra | 6.47 | 1.26 | -2.01 | -2.21 | |
| Il8rb | 4.65 | -4.75 | -3.21 | -2.93 | |
| Il10ra | -1.42 | 4.06 | -1.36 | -1.01 | |
| Others | Ifng | 3.58 | 2.59 | -3.21 | -2.93 |
| Tgfb1 | 1.74 | 2.63 | 3.62 | 3.03 | |
| Itgb2 | 1.88 | 4.58 | 1.00 | -1.10 | |
| Spp1 | 1.56 | 3.34 | -2.51 | -2.76 | |
| Tnfrsf1a | 5.47 | 3.14 | 2.74 | 1.84 | |
| Bcl6 | 3.39 | 1.84 | 3.28 | 2.78 | |
Fold changes more than 3 compared to data obtained from dark adapted mice are presented. The data was normalized to the housekeeping genes (Gusb, Gapdh, Actb, Hprt1 and Hsp90ab1).
Fold changes more than 10 are presented in bold text.
Minus signs (-) indicate reduced expression.
1
Mice were exposed to 10,000 lux white light for 30 min after 48 h of dark adaptation. Before such light exposure, pupils of mice were dilated. Mice were kept in the dark until evaluations.
2
RNA was purified from 16 retinas of 4-week-old mice.