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. 2014 Aug 6;9(8):e104247. doi: 10.1371/journal.pone.0104247

Figure 4. Dose-dependent effect of ethanol on the expression of CREB, P-CREB and PARIS in SH-SY5Y cells.

Figure 4

The SH-SY5Y cells were treated with various concentrations of ethanol for 48 hours. After the ethanol treatment, the cells were harvested and the cell lysate was analyzed to determine expression of total CREB, p-CREB and PARIS by Western blot analysis. β-actin was used for loading control. The protein density in each Western blot was quantitatively analyzed, and is shown in Panels B and C. Mean ± SE, n = 3, *P<0.05, **P<0.01 vs. control.