Table 1. The sequences of primers used for real-time quantitative PCR.

Gene symbol		Primer sequences	Product length (bp)
Gja1	Forward	5′-CCA TCC AAA GAC TGC GGA T-3′	138
	Reverse	5′-GTA ATT GCG GCA CGA GGA A-3′
Cox2	Forward	5′-TGG GTG TGA AGG GAA ATA AGG-3′	104
	Reverse	5′-CAT CAT ATT TGA GCC TTG GGG-3′
Nos2	Forward	5′-TCA GCC AAG CCC TCA CCT AC-3′	108
	Reverse	5′-CCA ATC TCT GCC TAT CCG TCT C-3′
Ptger4	Forward	5′-GTG GTG CTC ATC TGC TCC ATT C-3′	102
	Reverse	5′-CTG CAA ATC TGG GTT TCT GCT G-3′
Ngf	Forward	5′-AAT AGC TGC CCG AGT GAC AG-3′	124
	Reverse	5′-GTC TGA AGA GGT GGG TGG AG-3′
Trpv1	Forward	5′-TGT GGA GGT GGC AGA TAA CA-3′	100
	Reverse	5′-CTT CAG TGT GGG GTG GAG TT-3′
Chrm2	Forward	5′-CTG GAG CAC AAC AAG ATC CAG AAT-3′	70
	Reverse	5′-CCC CCT GAA CGC AGT TTT CAG T-3′
Chrm3	Forward	5′-GCA AGA CCT CTG ACA CCA ACT-3′	92
	Reverse	5′-AGC AAA CCT CTT AGC CAG CG-3′
Chrm4	Forward	5′-CGG CTA CTG GCT CTG CTA CGT CAA-3′	122
	Reverse	5′-CTG TGC CGA TGT TCC GAT ACT GG-3′
Gapdh	Forward	5′-GCA CAG TCA AGG CCG AGA AT-3′	131
	Reverse	5′-GCC TTC TCC ATG GTG GTG AA-3′