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. 2014 Jul 17;53(15):7812–7814. doi: 10.1021/ic501509x

Figure 2.

Figure 2

DNA binding of RhPt. Left: Competition titration of increasing concentrations of RhPt (0–50 μM) with 1 μM rac-[Rh(bpy)2chrysi]3+ on 1 μM 5′-[32P] labeled 17mer duplex DNA with a CC mismatch. Controls without irradiation (Øhν), and without Rh (ØRh) were included. RhPt inhibits photocleavage by [Rh(bpy)2chrysi]3+ at the mismatched site. Right: DMS footprinting of duplex DNA containing a CC mismatch. Lanes (left to right): Maxam–Gilbert sequencing (C+T; A+G); 3, DMS alone; 4, oxaliplatin (1 μM); 5, RhPt (1 μM); 6, RhPt (50 μM). Bands of high electrophoretic mobility indicate cleavage at guanine residues; covalent binding of platinum to guanine inhibits cleavage.