Figure 6.
Mechanisms of action of Taxol–octaarginine conjugates. (A) Tubulin polymerization assay. Conjugates were compared to free Taxol for the ability to polymerize free tubulin, as assessed by measuring increase in turbidity (absorbance at 350 nm).61 (B) Cell cycle assay. Conjugates were compared to free Taxol for the ability to kill cells through the same cell-cycle arrest mechanism.61 *Conditions under which octaarginine conjugates produce a significantly higher percentage of cells in G2/M phase than Taxol alone. Reproduced with permission from ref (61). Copyright 2008 National Academy of Sciences.