Figure 3.
Multicopy locus hybridization capture efficiency and specificity. The fractions of target DNA captured by HyCCAPP from the 25S rDNA locus, 5S rDNA locus, and X-element, as measured by locus-specific qPCR assays (Supporting Information) are shown in panels A, B, and C, respectively. Error bars represent the standard deviation of duplicate measurements. The first, second, and third bars in each plot show the fractions of DNA captured using the 25S rDNA capture oligonucleotides, the 5S rDNA capture oligonucleotides, and the X-element capture oligonucleotides, respectively. Numerical values in each case are shown.