Figure 4.

GAL1-10 locus hybridization capture efficiency and specificity. Ten capture oligonucleotides (30 nt) were designed for the GAL1-10 promoter region spanning a total length of about 1400 bp (a). The capture probes alternated spacing of 100 bp and 200 bp to avoid synchronicity with nucleosome repetition. Two qPCR assays were designed, one targeting a region near the start of the GAL10 gene (dark gray) and one targeting a region near the start of the GAL1 gene (light gray). HyCCAPP was performed using either capture oligonucleotides 1–5, capture oligonucleotides 6–10, or a scrambled oligonucleotide control (b). Error bars represent the standard deviation of duplicate measurements.