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. 2014 Aug 6;9(8):e104441. doi: 10.1371/journal.pone.0104441

Figure 5. Functional substitution by other OA receptors.

Figure 5

Transgenic expression of the OA receptors Octβ1R, Octβ3R, OAMB-K3 and OAMB-AS was driven by RS-GAL4 in the octβ2r’s oviduct epithelium. (A) Ovulation rescue. Ectopically expressed Octβ1R fully rescued the octβ2r’s ovulation phenotype while Octβ3R, OAMB-K3 and OAMB-AS yielded partial rescue (***, p<0.0001; **, p<0.005; ns, not significant; n = 18–34). (B) Fecundity rescue. Ectopic expression of Octβ1R fully restored fecundity whereas partial or no rescue was observed with ectopic expression of Octβ3R or OAMB-K3 and OAMB-AS, respectively (***, p<0.0001, n = 20–38). (C) RT-PCR analysis. RNA was isolated from dissected reproductive tissues of CS, octβ2r mutant females carrying RS-GAL4 alone, and octβ2r mutant females carrying RS-GAL4 and UAS- Octβ1R or UAS-Octβ3R for RT-PCR. The elevated levels of Octβ1R or Octβ3R PCR products were detectable in the octβ2r females carrying RS-GAL4 and UAS- Octβ1R or UAS-Octβ3R, respectively. Rp49 was used for an internal control.