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. Author manuscript; available in PMC: 2014 Aug 6.
Published in final edited form as: Plant Cell Environ. 2013 Dec 8;37(5):1192–1201. doi: 10.1111/pce.12227

Figure 4.

Figure 4

Essential metal ions increase PC transport activity in barley vacuoles. (a) Transport activity of PC2 complexes in the presence of essential metal ions. (b) Comparison of PC2 transport activity in barley vacuoles isolated from plants grown on soil with or without 50 μm CdCl2. Apo–PC2 and PC2 complexes (one-to-one ratio of PC2 and metal ion) with the indicated metal ions were added to transport buffer containing vacuoles. The final concentrations of apo–PC2 and PC2 complexes in the transport medium were 2.5 μm, and the reactions were incubated at room temperature for 15 min. The values indicate averages and standard errors from values obtained in the absence of Mg–ATP subtracted from those obtained in the presence of MgATP (n = 4, N = 2). *P < 0.05, **P < 0.01 (Student's t-test).