Skip to main content
. Author manuscript; available in PMC: 2015 Jul 1.
Published in final edited form as: Exp Neurol. 2014 Apr 18;257:186–204. doi: 10.1016/j.expneurol.2014.04.008

Table 4.

Histological methods overview.

Group Animal # Histological preparation Stains Section sets
Transection only (n = 9) 6, 8, 15, 17, 19, 20, 27, 29, 30

  • Horizontal sections through lesion block

  • GFAP + Hoescht

  • Neurofilament + Hoescht

  • 1/180 µm sets through lesion for each immunosrain.
Transection + transplant (n = 14) 1, 2, 3, 9, 11, 12, 13, 16, 18, 22,
23, 25, 26, 28

  • Horizontal sections through lesion block

  • Cross-sections at C2, C4, C8, T6, T8, T10, T12, Ll/2 and L4

  • Coronal sections through brain

  • GFP only

  • GFP + GFAP + Hoescht

  • GFP + Neurofilament + Hoescht

  • 5-HT + Hoescht (rat # 12, 22, 23, 26, 31)

  • 1/180 µm sets through lesion for each immunosrain.

  • Representative cross sections for GFP only.
Transection + transplant +
  re-transection (n = 2)		 7, 10

  • Horizontal sections through lesion block

  • Cross-sections at C2, C4, C8, T6, T8, T10, T12, Ll/2 and L4

  • Coronal sections through brain

  • GFP only

  • GFP + GFAP + Hoescht

  • 1/180 µm sets through lesion for GFP + GFAP immunosrain.

  • Representative cross sections for GFP only.
Transection + transplant +
  BDA (n = 4)		 5, 24, 31, 32

  • Horizontal sections through lesion block

  • Cross-sections at C2, C4, C8, T6, T8, T10, T12, Ll/2 and L4

  • Coronal sections through brain

  • GFP only

  • GFAP only

  • GFP + GFAP + Hoescht

  • GFP + Neurofilament + Hoescht

  • BDA

  • GFP + BDA

  • 5-HT + Hoescht

  • 1/180 µm sets through lesion for each immunostain.

  • Representative cross sections for GFP only and GFP + BDA.