Table 2. Frequencies of species within Anopheles gambiae complex and of molecular forms of Anopheles gambiae s.s. in the two transects.

Transects/Sampling sites	Collected	Sibling species identification			Molecular forms identification
		N	An. arabiensis	An. gambiae s.s.	N	M-form	S-form	M/S hybrids
			%	%		%	%	Obs.	Exp.	Fis
Transect 1
Neteboulou	166	146	60.3	39.7	58	19.0	79.3	1.7	31.8	+0.95
Tourema	231	146	73.3	26.7	39	23.0	74.4	2.6	36.9	+0.93
Gouloumbou	435	300	61.0	39.0	117	16.2	78.6	5.2	30.5	+0.83
Afia	124	94	45.7	54.3	51	11.8	86.2	2.0	22.2	+0.91
Temento Malede	362	156	73.7	26.3	41	19.5	78.0	2.5	32.9	+0.93
Sare Sidy	205	104	46.2	53.8	56	7.1	89.3	3.6	16.3	+0.78
Sankagne	377	174	74.1	25.9	45	26.7	66.6	6.7	42.0	+0.84
Dialiko	126	120	50.8	49.2	59	22.0	78.0	0.0	34.4	+1
Nguene	416	159	61.0	39.0	62	21.0	79.0	0.0	33.1	+1
Koar	310	256	41.0	59.0	151	15.9	83.4	0.7	27.2	+0.98
Saal	324	165	62.4	37.6	62	30.6	69.4	0.0	42.5	+1
Transect 2
Tamba Soce	50	48	60.4	39.6	19	0.0	94.7	5.3	5.1	–0.03*
Djounkore Mafing	356	213	50.2	49.8	106	2.8	92.5	4.7	9.8	+0.52
Madina Dian	69	66	63.6	36.4	24	8.3	87.5	4.2	18.7	+0.78
Missirah	14	14	64.3	35.7	5	20.0	80.0	0.0	32.0	+1
Barkeyel	180	88	62.5	37.5	33	12.1	87.9	0.0	21.3	+1
Gourel Ndiapalde	100	85	15.3	84.7	72	5.6	93.0	1.4	11.7	+0.88
Bira	74	61	41.0	59.0	36	5.6	94.4	0.0	10.5	+1
Badi	331	226	62.4	37.6	85	29.4	70.6	0.0	41.5	+1
Wassadou	348	162	35.8	64.2	104	6.7	92.3	1.0	13.4	+0.93

% = Percentage, Obs. = observed, Exp. = expected, Fis = inbreeding coefficient calculated according to Weir and Cockerham [37], Fis<0 indicate an excess of heterozygotes, Fis>0 denote heterozygotes deficiency, *no significant deviation from Hardy–Weinberg expectations (P>0.05).