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. 2014 Aug 6;9(8):e104629. doi: 10.1371/journal.pone.0104629

Figure 1. Melting temperature curve profiles produced by male and female samples in a HRM analysis.

Figure 1

HRM analysis starts with a PCR amplification of the amelogenin gen. The amplification is in the presence of EvaGreen a saturating dye which presents high fluorescence when bound to dsDNA. Amplification is followed by a High Resolution Melting analysis (HRM) in the LightCycler 480 Real-Time PCR Instrument (Roche Applied Science). A dissociation curve analysis displays the different melting temperature of products from the X and Y chromosome.