Figure 7. Dye release normalized to the change in tryptophan fluorescence quenching measured for β₂m with BMP-containing LUVs at pH 4.5.

The ratio of % dye release per membrane interaction detected via a change in tryptophan quenching observed in the absence or presence of lipid. β₂m monomers, fragmented and unfragmented fibrils (6 µM monomer equivalent concentration) were incubated in Assay Buffer pH 4.5 with LUVs (5 µM lipid) comprising 36 POPC: 20 POPE: 7 SM: 25 cholesterol (mol/mol) plus 0 mol % (blue), 12 mol % (red) or 50 mol % (green) BMP for 10 min at 37°C. Error bar represent 1 S.E.