Fig. 1.

Co-cultured neural stem cells (NSCs) and the adult brain slices in a transwell system. (A) Co-culture the brain slices with or without NSCs separated by a membrane. (B) Representative images show 1-week-old neurospheres, bar: 50 μm. (C) Neurons derived by plating neurospheres and allowing them to grow without growth factors for 1 week, bar: 20 μm. (D) Cells migrating out of a neurosphere that had been allowed to attach to a substrate 7 days (nestin staining). After 7 days in serum-containing medium, many cells had differentiated towards several neural cell types (E and F), bar: 50 μm. (E) Cells expressing microglia maker IBA-1, bar: 10 μm. (F) Cells expressing neuron maker NeuN, bar: 10 μm.