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. 2014 Apr 13;18(7):1300–1312. doi: 10.1111/jcmm.12281

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© 2014 The Authors. Journal of Cellular and Molecular Medicine published by John Wiley & Sons Ltd and Foundation for Cellular and Molecular Medicine.

This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

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Fig. 5 — Neural stem cells (NSCs) induced microglial activation via TLR9 and ERK1/2. (A) The real-time PCR analysis of the TLR9, TLR2 and TLR4 mRNA expression level in the slices co-cultured with or without NSCs. (B) p-ERK1 phosphorylation level in the slices. (lanes 1, 3, 5, 7 and 9, NSCs-treated group; lanes 2, 4, 6, 8 and 10, mock-treated group). (C) TLR9 mRNA level positively correlated with IBA-1 mRNA level. (D) p-ERK1 protein level positively correlated with IBA-1 protein level. Results are shown as means ± SD (n = 5).