Table 1.

Comparison of the average cell numbers between slices co-cultured with neural stem cells and control slices on different cultured days

Group (±SD)	Treatment	Viable cells	Leaky cells	Dead cells	Total cells
DIV 3	NSCs	2.57 ± 1.31***	1.13 ± 0.79***	1.61 ± 1.36***	5.31 ± 2.08***
DIV 3	Mock-treated	0.97 ± 0.35	2.73 ± 0.60	4.07 ± 1.57	7.77 ± 1.73
DIV 7	NSCs	2.69 ± 0.67***	0.85 ± 0.24***	0.59 ± 0.24***	4.13 ± 0.67***
DIV 7	Mock-treated	0.80 ± 0.23	2.54 ± 0.56	3.91 ± 0.74	7.25 ± 0.51
DIV 10	NSCs	2.76 ± 0.78***	0.59 ± 0.34***	0.20 ± 0.32***	3.55 ± 1.31***
DIV 10	Mock-treated	0.51 ± 0.21	2.33 ± 0.99	4.43 ± 1.43	7.27 ± 1.15

NSCs: slices co-cultured with neural stem cells, Mock-treated: slices cultured without neural stem cells. ***P < 0.001 indicates significant difference between NSCs-treated and mock-treated groups at the same cultured days. The cell numbers are shown as per mm³ (×1000), DIV: day in vitro.