Table 2.
Effect of TLR4 blocking on cytokine secretion by HPV vaccine-stimulated IL-15 DC
| No stimulus (pg/ml) | LPS | HPV vaccine | |||
|---|---|---|---|---|---|
| No CLI095 (pg/ml) | With CLI095 (pg/ml) | No CLI095 (pg/ml) | With CLI095 (pg/ml) | ||
| Typical Th1-polarizing | |||||
| IFN-γ | 17 ± 6 | 2735 ± 1121 | 38 ± 15** | 781 ± 368 | 41 ± 15* |
| IL-12p70 | 0 ± 0 | 28 ± 8 | 0 ± 0 | 0 ± 0 | 0 ± 0 |
| IL-2 | 1 ± 1 | 4 ± 1 | 1 ± 1 | 2 ± 1 | 1 ± 0 |
| Typical Th2-polarizing | |||||
| IL-4 | 1 ± 0 | 3 ± 1 | 1 ± 0 | 1 ± 0 | 0 ± 0 |
| IL-5 | 0 ± 0 | 5 ± 1 | 1 ± 0 | 2 ± 1 | 1 ± 0 |
| IL-10 | 2 ± 1 | 659 ± 614 | 33 ± 29 | 125 ± 115 | 1 ± 0 |
| IL-13 | 2 ± 2 | 16 ± 4 | 2 ± 2 | 7 ± 2 | 0 ± 0 |
| Pro-inflammatory | |||||
| IL-1β | 61 ± 46 | 1452 ± 84 | 170 ± 38** | 697 ± 322 | 109 ± 15* |
| TNF-α | 37 ± 12 | 8443 ± 226 | 345 ± 273*** | 3430 ± 1699 | 447 ± 273* |
CLI095, TLR4 inhibitor; HPV, human papillomavirus; IFN, interferon; IL, interleukin; Th, T helper; TLR4, Toll-like receptor 4; TNF, tumor-necrosis factor; LPS, lipopolysaccharide.
*P < 0.05, **P < 0.01, ***P < 0.001 (compared between CLI095 and no CLI095 added; n = 2).