Fig. 2.

Curcumin alleviates sinusoidal angiogenesis in rats with CCl₄-caused fibrosis. Rats were grouped: group 1, vehicle control (no CCl₄, no treatment); group 2, model group (with CCl₄, no treatment); group 3, curcumin-treated group (100 mg/kg + CCl₄); group 4, curcumin-treated group (200 mg/kg + CCl₄); group 5, curcumin-treated group (400 mg/kg + CCl₄). (A) Liver sections were stained with immunofluorescence by using antibodies against CD31, vWF, CD34, VEGF-R2, PDGF-βR, and VEGF. (B) ELISA measurement of VEGF levels in liver and blood. (C) Real-time PCR analyses of HIF-1α, VEGF-R2, PDGF-βR, and VEGF in liver tissues. (D) Western blot analyses of liver proteins with densitometry. For the statistics of each panel in this figure, ^#P < 0.05 versus group 1, ^##P < 0.01 versus group 1, *P < 0.05 versus group 2, **P < 0.01 versus group 2, n = 6.