Fig. 3.

Curcumin does not affect endothelial cells and aortic ring vascularization in vitro. (A and B) LSECs were treated with DMSO (0.02%, w/v), imatinib and curcumin for 24 or 48 hrs. Cell viability was evaluated by MTS assay (A). Lactate dehydrogenase activity in supernatant was assessed (B). (C) LSECs were incubated with DMSO (0.02%, w/v), imatinib and curcumin on matrigel for 24 hrs. Tubulogenesis was visualized and quantified. (D) Explanted aortic rings were placed on matrigel and subjected to DMSO (0.02%, w/v), imatinib and curcumin for 7 days (n = 6). The histogram shows quantification of aortic sprouts specifically from the endothelial cell lumen. Values are represented as arbitrary units. For the statistics of each panel in this figure, *P < 0.05 versus DMSO, **P < 0.01 versus DMSO.