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. 2014 Apr 30;18(7):1392–1406. doi: 10.1111/jcmm.12286

Fig. 5.

Fig. 5

Curcumin interrupts PDGF-βR/FAK/RhoA pathway linking to inhibited HSC invasion and vascularization. (A) HSC invasion for 24 hrs was evaluated by Boyden chamber assay with DMSO (0.02%, w/v) and curcumin in the upper well and PDGF in the lower well. (B–D) HSCs were treated with DMSO (0.02%, w/v), imatinib (B), Y15 (C), or curcumin (D) for 24 hrs prior to PDGF stimulation for an additional 3 hrs. Western blot analyses of FAK/RhoA signals. (E) HSC invasion for 24 hrs was evaluated by Boyden chamber assay with DMSO (0.02%, w/v), imatinib (10 μM), Y15 (10 μM), fasudil (10 μM) and curcumin (20 μM) in the upper well and PDGF in the lower well. (F) HSCs were incubated with DMSO (0.02%, w/v), PDGF (20 ng/ml), imatinib (10 μM), Y15 (10 μM), fasudil (10 μM) and curcumin (20 μM) on matrigel for 24 hrs. Tubulogenesis was visualized and quantified. For the statistics of each panel in this figure, #P < 0.05 versus DMSO, *P < 0.05 versus DMSO + PDGF.