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. 2014 Jul 30;3:395. doi: 10.1186/2193-1801-3-395

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© Fazel et al.; licensee Springer. 2014

This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited.

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Restriction analysis of pPICZαA-UOX construct. A: Schematic representation of pPICZαA-UOX construct map. B: Restriction mapping of pPICZαA-UOX plasmid. Lane 1: Size marker. Lane 2: Fragments created by EcoRV/HindIII treatment. Two bands of 2602 and 1864 bp are seen. Lane 3: Fragments created by BamHI/HindIII digestion of the construct with size of 2788 and 1678 bp. Lane 4: The restriction fragments created by XbaI/XhoI digestion. Two fragments of 3515 and 951 bp are present. Lane 5: XbaI linearized construct with a size of 4466.