Table 1. Primary antibody information.
Antibody | Cell type specific for | Source and type of antibody | Vendor |
---|---|---|---|
BrdU (bromodeoxyuridine) | Proliferating cells | Rat monoclonal | abcam |
Ki-67 | Rabbit polyclonal | DAKO | |
Nestin | Neural progenitors | Mouse monoclonal | DSHB |
TuJ1 (βIII-tubulin) | Young neurons | Mouse monoclonal | R&D Systems |
MAP2ab (microtubule associated protein 2ab) | Mature neurons | Mouse monoclonal | Sigma |
RIP (receptor interacting protein) | Oligodendrocytes | Mouse monoclonal | DSHB |
GFAP (glial fibrillary acidic protein) | Astrocytes | Mouse monoclonal | Lab Vision |
Brn3a (brain-specific hoeobox/POU domain protein 3a) | Ganglion cells | Mouse monoclonal | Millipore |
Calretinin | Neurons in INL | Rabbit polyclonal | Millipore |
PKCα (protein kinase C alpha) | Rod bipolar cells | Mouse monoclonal | DSHB |
Pax6 (paired box gene 6) | Amacrine cells | Mouse monoclonal | DSHB |
Recoverin | Photoreceptors | Rabbit polyclonal | Millipore |
Rho1D4 (rhodopsin 1D4) | Photoreceptors | Mouse monoclonal | abcam |
CRALBP (cellular retinaldehyde-binding protein) | Retinal glial cells | Rabbit polyclonal | Thermo Scientific |
GS (Glutamine synthetase) | Retinal glial cells | Mouse monoclonal | Millipore |