Table 4.
Details of Applied Biosystems Primers ( n = 18), including the housekeeping gene, ALG9
| SI-NEN Biomarker or housekeeping gene | NCBI chromosome location | UniGene ID | RefSeq | Amplicon produced using forward and reverse primers | ||
|---|---|---|---|---|---|---|
| Symbol | Name | Length | Exon boundary | |||
| ALG9* | Asparagine-linked glycosylation 9, alpha-1,2-mannosyltransferase homolog | Chr. 11–111652919 - 111742305 | Hs.503850 | NM_024740.2 | 68 | 4-5 |
| ADCY2 | Adenylate cyclase 2 (brain) | Chr.5: 7396343 - 7830194 | Hs.481545 | NM_020546.2 | 81 | 22-23 |
| AKT3 | v-akt murine thymoma viral oncogene homolog 3 | Chr.1: 243651535 – 244006886 | Hs.498292 | NM_001206729.1 | 100 | 11-12 |
| APLP1 | Amyloid beta (A4) precursor-like protein 1 | Chr.19: 36359401 – 36370699 | Hs.74565 | NM_001024807.1 | 142 | 11-12 |
| BEX1 | Brain expressed, X-linked 1 | Chr.X: 102317581 – 102319168 | Hs.334370 | NM_018476.3 | 62 | 2-3 |
| CEBPA | CCAAT/enhancer binding protein (C/EBP), alpha | Chr.19: 33790840 - 33793430 | Hs.740432 | NM_004364.3 | 77 | 1-1 |
| CPE | carboxypeptidase E | Chr.4: 166300097 - 166419482 | Hs.75360 | NM_001873.2 | 106 | 7-8 |
| INSM1 | Insulinoma-associated 1 | Chr.20: 20348765 - 20351593 | Hs.89584 | NM_002196.2 | 72 | 1-1 |
| NEUROD1 | Neuronal differentiation 1 | Chr.2: 182541194 - 182545381 | Hs.574626 | NM_002500.4 | 110 | 2-2 |
| NKX2-2 | NK2 homeobox 2 | Chr.20: 21491648 - 21494664 | Hs.516922 | NM_002509.3 | 114 | 1-2 |
| PCSK1 | Proprotein convertase subtilisin/kexin type 1 | Chr.5: 95726040 - 95768985 | Hs.78977 | NM_000439.4 | 96 | 13-14 |
| PNMA2 | paraneoplastic Ma antigen 2 | Chr.8: 26362196 - 26371483 | Hs.591838 | NM_007257.5 | 60 | 3-3 |
| SCG2 | Secretogranin II | Chr.2: 224461658 – 224467121 | Hs.516726 | NM_003469.4 | 69 | 1-2 |
| SCG3 | Secretogranin III | Chr.15: 51973550 - 52013223 | Hs.232618 | NM_001165257.1 | 92 | 5-6 |
| SCG5 | Secretogranin V | Chr.15: 32933870 - 32989298 | s.156540 | NM_001144757.1 | 84 | 5-6 |
| SCN3A | Sodium channel, voltage-gated, type III, alpha subunit | Chr.2: 165944030 – 166060577 | Hs.435274 | NM_001081676.1 | 71 | 12-13 |
| SDHD | Succinate dehydrogenase complex, subunit D, integral membrane protein | Chr.11: 111957571 – 111966518 | Hs.356270 | NM_003002.2 | 187 | 4-4 |
| ST18 | Suppression of tumorigenicity 18 (breast carcinoma) (zinc finger protein) | Chr.8: 53023392 – 53322439 | Hs.655499 | NM_014682.2 | 69 | 22-23 |
*ALG9 = housekeeping gene.