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. 2014 Jul 22;15(1):618. doi: 10.1186/1471-2164-15-618

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© Pessina et al.; licensee BioMed Central Ltd. 2014

This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Transcriptional variation of three apple MLO genes following inoculation with P. leucotricha. Transcript abundances of three MLO genes in leaves of the apple cultivars ‘Braeburn’, ‘Golden Delicious’ and ‘Gala’, following powdery mildew (PM) inoculation. Here we show only MLO genes that were more than one time significantly up or down regulated following PM inoculation at one of the four time points examined (4, 6, 8 and 24 hpi). The set of results of all investigated genes is shown in Additional file 1: Figure S1. Each bar shows the average of four to eight biological replicates. The Ct values have been normalized for three reference genes: actin, ubiquitin and elongation factor 1. Statistical significance was determined with a t-test for each individual pair of inoculated and non-inoculated samples at each time point. The error bars show standard errors of the means. Significant differences between inoculated samples and control samples are indicated with an asterisk (P < 0.05).