Table 1.
qPCR validation of selected transcripts exhibiting differential accumulation in microarray
| Microarray | qPCR | |||||
|---|---|---|---|---|---|---|
| Gene | Comparison | Fold change (Log 2) | P-val (Bonferroni) | Fold change (Log 2) | Standard error | Gene description |
| Bradi1g75310 | Fg don + vs Tween | 6.93 | 0.00 | 14.40 | 0.26 | Cytochrome P450 monooxygenase |
| Fg don - vs Tween | 0.77 | 1.00 | 6.28 | 0.40 | ||
| Fg don + vs Fg don - | 6.16 | 0.00 | 8.12 | 0.26 | ||
| Bradi3g22880 | Fg don + vs Tween | 7.44 | 0.00 | 10.24 | 0.08 | MATE efflux |
| Fg don - vs Tween | 1.36 | 0.18 | 2.26 | 0.11 | ||
| Fg don + vs Fg don - | 6.08 | 0.00 | 7.97 | 0.08 | ||
| Bradi2g41010 | Fg don + vs Tween | 9.30 | 0.00 | 12.93 | 0.15 | Unknown |
| Fg don - vs Tween | 4.80 | 0.00 | 3.84 | 0.25 | ||
| Fg don + vs Fg don - | 4.51 | 0.00 | 9.08 | 0.15 | ||
| Bradi2g35930 | Fg don + vs Tween | 8.19 | 0.00 | 10.09 | 0.07 | Glutathione S-transferase GSTF1-like |
| Fg don - vs Tween | 4.15 | 0.00 | 3.46 | 0.45 | ||
| Fg don + vs Fg don - | 4.05 | 0.00 | 6.63 | 0.07 | ||
| Bradi4g39950 | Fg don + vs Tween | 2.38 | 0.00 | 3.12 | 0.19 | NADP-dependent alkenal double bond reductase P1 |
| Fg don - vs Tween | 5.68 | 0.00 | 6.05 | 0.28 | ||
| Fg don + vs Fg don - | -3.29 | 0.00 | -2.93 | 0.19 | ||
| Bradi4g05040 | Fg don + vs Tween | 2.25 | 0.00 | 3.35 | 0.06 | PR10 |
| Fg don - vs Tween | 7.48 | 0.00 | 8.42 | 0.27 | ||
| Fg don + vs Fg don - | -5.22 | 0.00 | -5.08 | 0.06 | ||
| Bradi4g36870 | Fg don + vs Tween | 3.69 | 0.00 | 4.75 | 0.05 | Ornithine decarboxylase |
| Fg don - vs Tween | 0.90 | 1.00 | 1.59 | 0.50 | ||
| Fg don + vs Fg don - | 2.77 | 0.00 | 3.16 | 0.05 | ||
| Bradi4g36810 | Fg don + vs Tween | 5.06 | 0.00 | 10.14 | 0.28 | Ornithine decarboxylase |
| Fg don - vs Tween | 1.37 | 0.15 | 5.55 | 0.26 | ||
| Fg don + vs Fg don - | 3.69 | 0.00 | 4.59 | 0.28 | ||
| Bradi1g50067 | Fg don + vs Tween | 3.63 | 0.00 | 5.23 | 0.16 | Arginine decarboxylase |
| Fg don - vs Tween | 1.16 | 1.00 | 1.58 | 0.31 | ||
| Fg don + vs Fg don - | 2.46 | 0.00 | 3.65 | 0.16 | ||
| Bradi3g47110 | Fg don + vs Tween | 5.86 | 0.00 | 11.99 | 0.34 | Phenylalanine ammonia-lyase |
| Fg don - vs Tween | 0.32 | 1.00 | 5.59 | 0.59 | ||
| Fg don + vs Fg don - | 5.53 | 0.00 | 6.41 | 0.34 | ||
| Bradi3g53227 | Fg don + vs Tween | 3.56 | 0.00 | 6.94 | 0.14 | 3-hydroxy-3-methyl-glutaryl-CoA reductase |
| Fg don - vs Tween | 0.17 | 1.00 | 1.77 | 0.15 | ||
| Fg don + vs Fg don - | 3.40 | 0.00 | 5.17 | 0.14 | ||
| Bradi2g13360 | Fg don + vs Tween | 4.10 | 0.00 | 5.69 | 0.14 | Mevalonate kinase |
| Fg don - vs Tween | 1.93 | 0.00 | 2.26 | 0.36 | ||
| Fg don + vs Fg don - | 2.17 | 0.00 | 3.43 | 0.14 | ||
| Bradi5g02780 | Fg don + vs Tween | 2.41 | 0.00 | 4.87 | 0.16 | UDP-glycosyltransferase |
| Fg don - vs Tween | 1.16 | 1.00 | 2.66 | 0.36 | ||
| Fg don + vs Fg don - | 1.25 | 1.00 | 2.21 | 0.16 | ||
| Bradi5g03300 | Fg don + vs Tween | 7.46 | 0.00 | 12.01 | 0.16 | UDP-glycosyltransferase |
| Fg don - vs Tween | 0.88 | 1.00 | 2.92 | 0.47 | ||
| Fg don + vs Fg don - | 6.59 | 0.00 | 9.08 | 0.16 | ||
| Bradi5g03370 | Fg don + vs Tween | 6.61 | 0.00 | 9.51 | 0.19 | UDP-glycosyltransferase |
| Fg don - vs Tween | 2.09 | 0.00 | 3.29 | 0.44 | ||
| Fg don + vs Fg don - | 4.52 | 0.00 | 6.22 | 0.19 | ||
| Bradi5g03380 | Fg don + vs Tween | 6.72 | 0.00 | 12.46 | 0.22 | UDP-glycosyltransferase |
| Fg don - vs Tween | 1.52 | 0.01 | 4.36 | 0.73 | ||
| Fg don + vs Fg don - | 5.20 | 0.00 | 8.10 | 0.22 | ||
| Bradi5g03390 | Fg don + vs Tween | 4.34 | 0.00 | 6.23 | 0.08 | UDP-glycosyltransferase |
| Fg don - vs Tween | 1.86 | 0.00 | 2.15 | 0.44 | ||
| Fg don + vs Fg don - | 2.49 | 0.00 | 4.08 | 0.08 | ||
| Bradi5g03400 | Fg don + vs Tween | 1.22 | 1.00 | 2.51 | 0.13 | UDP-glycosyltransferase |
| Fg don - vs Tween | 1.37 | 0.14 | 2.01 | 0.10 | ||
| Fg don + vs Fg don - | -0.16 | 1.00 | 0.50 | 0.13 | ||
| Bradi2g04720 | Fg don + vs Tween | 5.41 | 0.00 | 8.76 | 0.20 | UDP-glycosyltransferase |
| Fg don - vs Tween | 3.22 | 0.00 | 5.47 | 0.24 | ||
| Fg don + vs Fg don - | 2.19 | 0.00 | 3.29 | 0.20 | ||
| Bradi2g04760 | Fg don + vs Tween | 4.71 | 0.00 | 7.25 | 0.19 | UDP-glycosyltransferase |
| Fg don - vs Tween | 1.04 | 1.00 | 2.57 | 0.26 | ||
| Fg don + vs Fg don - | 3.67 | 0.00 | 4.68 | 0.19 |