Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2014 Jul 28;15(1):630. doi: 10.1186/1471-2164-15-630

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© Wang et al.; licensee BioMed Central Ltd. 2014

This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

PMC Copyright notice

The exon trapping vector pSPL3 used to assay SNP function. (A) The pSPL3 vector contains SD (splice donor) and SA (splice acceptor) sites that operate as exons, and a functional intron, with transcription beginning following the SV40 promoter and ending at the LPAS (late poly (A) signal). Wild pSPL3-W and mutant pSPL3-M plasimds containing 914 bp of intron 8, exon 9 and 453 bp of intron 9 and harboring either the C or T alleles were separately cloned into the EcoRI and XhoI cloning sites of the pSPL3 vector. (B) Agarose gel electrophoresis of RT-PCR products. SD6 and SA2 primers were designed for RT-PCR amplification of cDNA sequences generated by transfected 293 T cells. Lane1: Marker;DNA Marker 600 (TIANGEN, China); Lane2: 263 bp; Lane3: 365 bp (263 bp + 54 bp + 48 bp) and 317 bp (263 bp + 54 bp); Lane 4: 317 bp (263 bp + 54 bp). MCS: multiple cloning sites.