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. 2014 Jul 16;2014:532161. doi: 10.1155/2014/532161

Figure 6.

Figure 6

IL-8 induced drug resistance against doxorubicin in MDA-MB-231 cells. (a) Cells were treated by 200 nM doxorubicin for 24 hours with or without pretreatment of human recombinant IL-8 (50 or 100 ng/mL) for 24 hours and cell viability was examined by performing crystal violet staining. *P < 0.05 to control group; # P < 0.05 to doxorubicin-treated group. (b) IL-8 neutralizing antibody (5 μg/mL) antagonized MSC-ad conditioned medium-induced doxorubicin (200 nM) resistance. IgG isotype control antibody was used as negative control. *P < 0.05 to MSCM with doxorubicin-treated group; # P < 0.05 to CM with doxorubicin-treated group. Graphs showed mean ± SEM of three independent experiments. Doxo, doxorubicin; MSCM, mesenchymal stem cell medium (fresh MSC culture medium); CM, conditioned medium from adipose-derived mesenchymal stem cells (MSC-ad); NeuAb, IL-8 neutralizing antibody.