Fig. 5.

Effect on cell apoptosis from combined treatment of 5-FU and DGLA׳s radical derivatives. A: Cell apoptosis analysis of HCA-7 colony 29 cells treated with 5-FU (0.5 mM) alone or 5-FU combined with DGLA׳s radical derivatives (1.0 μM) for 48 h; B: p53 and pro-caspase 9 expression in Western blot upon different treatments for 48 h; and C: Quantification of p53 and pro-caspase 9 expression rate compared to control (treated with vehicle). The protein expression rate in individual treatments was calculated as the ratio to beta-actin, and the rate in the control group was normalized to 1. (^⁎p<0.05 from n≥3).