Table 1.
Profile of PUFAs, PGs, and free radicals (trapped by POBN) from HCA-7 cells treated with DGLA (100 μM). PUFAs and PGs from 2×106 cells treated with DGLA (100 μM) were measured via LC/MS analysis using internal standards, including AA-d8, DGLA-d6, PGE1-d4 and PGE2-d9. The formation of free radicals (trapped by POBN) from 2×106 cells treated with POBN (20 mM) and DGLA (100 μM) was quantified by LC/MS analysis. Deuterated-POBN (d9-POBN) was used as an internal standard for hydroxylamine quantification [22–24,27]. Data are represented as mean±SD, n=3.
| Time (h) | [PUFAs] (nM) |
[PGs] (nM) |
[DGLA free radicals] (nM) trapped by POBN as hydroxylamine |
||||
|---|---|---|---|---|---|---|---|
| DGLA | AA | PGE1 | PGE2 | HEX | HTA | 8-HOA | |
| 12 | 562.6±9.556 | 301.0±55.77 | 86.57±13.55 | 85.13±9.277 | 79.24±5.685 | 7.282±0.793 | 7.142±0.866 |
| 24 | 541.4±7.938 | 304.0±60.29 | 145.9±8.712 | 104.5±26.58 | 65.69±4.531 | 5.800±1.10 | 8.488±0.386 |
| 48 | 30.93±6.474 | 69.74±8.040 | 71.36±2.627 | 113.2±21.26 | 59.47±3.850 | 4.654±0.693 | 6.978±0.750 |