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Analysis of expressed products of α-β fusion gene in E.coli by SDS-PAGE (A) and Western blot (B) In A, lane 1: protein markers (97400, 66200, 43000, 31000, 20100, 14400); lanes 2, 3: BL21(DE3)( pZCPAB); lane 4: BL21(DE3)(pBV221); In B, lane 1: BL21(DE3)(pZCPAB)/α; lane 2: BL21(DE3)(pZCPAB)/α+β; lane 3: BL21(DE3)( pZCPAB)/β; lanes 4, 5: BL21(DE3)(pBV221).
