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. Author manuscript; available in PMC: 2014 Aug 7.
Published in final edited form as: Nat Protoc. 2014 Jan 23;9(2):375–395. doi: 10.1038/nprot.2014.017

Table 3. Scaling Up Phospho-Antibody Purification.

Serum to be used Peptide (mg) Maleimide activated BSA (mg) Amount of Cyanogen Bromide Bed Volume Obtained Fractions collected (1ml)
1ml 2mg 2mg 0.3g 1ml 10
2ml 2mg 2mg 0.3g 1ml 20
2-4ml 4mg 4mg 0.6g 2ml 20-40
3-6ml 6mg 6mg 0.9g 3ml 30-50