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. 2014 Aug 1;8:19. doi: 10.1186/1754-1611-8-19

Table 1.

Bacterial strains and plasmids used in this study

Strain Relevant description Reference
ARY023
rcnA::uidA-kan
[16]
HYD720
ΔnikA-kan
[24]
MG1655
F- λ-
 
S57
MG1655/pIG50
this study
S59
MG1655 nikA
this study
S63
S59/pIG50
this study
SCC1 = S29
MG1655 (PA1/04/03 gfpmut3*Cm)
[25]
S48
MG1655 (PA1/04/03 gfpmut3*Cm) rcnA::uidA-kan,
this study
S61 “NiCo buster”
S48/pIG50
this study
1137 = S71
MG1655 malT::Tn10 ompR234 csgA::uidA-kan
[26]
Plasmids
Relevant description
Reference
pSB1C3
pUC19-derived pMB1 (copy number: 100–300) CmR
[27]
pSB1T3
pUC19-derived pMB1 (copy number: 100–300) TetR
[28]
pIG2
rcn-csgBAEFG inserted at sites EcoRI/PstI of pUC57
[22]
pIG49
Ptac-nicoTB in pSB1C3
this study
pIG50 Ptac-nicoTB-rcn-csgBAEFG in pSB1T3 this study