Table 1.
Bacterial strains and plasmids used in this study
| Strain | Relevant description | Reference |
|---|---|---|
| ARY023 |
rcnA::uidA-kan |
[16] |
| HYD720 |
ΔnikA-kan |
[24] |
| MG1655 |
F- λ- |
|
| S57 |
MG1655/pIG50 |
this study |
| S59 |
MG1655 nikA |
this study |
| S63 |
S59/pIG50 |
this study |
| SCC1 = S29 |
MG1655 (PA1/04/03 gfpmut3*Cm) |
[25] |
| S48 |
MG1655 (PA1/04/03 gfpmut3*Cm) rcnA::uidA-kan, |
this study |
| S61 “NiCo buster” |
S48/pIG50 |
this study |
| 1137 = S71 |
MG1655 malT::Tn10 ompR234 csgA::uidA-kan |
[26] |
|
Plasmids |
Relevant description |
Reference |
| pSB1C3 |
pUC19-derived pMB1 (copy number: 100–300) CmR |
[27] |
| pSB1T3 |
pUC19-derived pMB1 (copy number: 100–300) TetR |
[28] |
| pIG2 |
rcn-csgBAEFG inserted at sites EcoRI/PstI of pUC57 |
[22] |
| pIG49 |
Ptac-nicoTB in pSB1C3 |
this study |
| pIG50 | Ptac-nicoTB-rcn-csgBAEFG in pSB1T3 | this study |