TABLE 1.

Differences and similarities between dark-adapted mice from different Rdh12 genetic backgrounds

	Rdh12+/+	Rdh12+/−	Rdh12−/−
	pmol/eye	pmol/eye	pmol/eye
Rhodopsina
6-week-old	480.3 ± 25.8	493.3 ± 25.9	487.4 ± 28.8
	Rdh12+/+	Rdh12+/−	Rdh12−/−
	pmol/eye	pmol/eye	pmol/eye
A2Eb
6-week-old	NDc	ND	ND
6-month-old	5.89 ± 0.48	8.11 ± 1.13	10.51 ± 1.78
12-month-old	11.00 ± 0.17	17.74 ± 0.20	22.03 ± 1.10
	Rdh12+/+	Rdh12+/−	Rdh12−/−
	min		min
Meta II decayd
6-week-old	30.6		27.8
Expression level (retina)e	Rdh12+/+	Rdh12−/−	Rdh8−/−
	Relative quantification	Relative quantification	Relative quantification
RDH12	1	0.00	1.00
RDH8	1	1.01	0.00

^aMice were dark-adapted for more than 48 h. The results are presented with S.E., and n was between 3 and 5. Rhodopsin and retinoids were measured as described under “Materials and Methods.”

^bA2E was measured as described under “Materials and Methods.” The results are presented with S.E., and n was between 3 and 5.

^cND, not detected.

^dMeta II decay (n = 3) was examined as described under “Materials and Methods.”

^eQuantitative PCR was carried out in the DNA Sequencing and Real-time PCR Core (Department of Orthopedics, Case Western Reserve University).