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. 2013 Oct 8;8(1):83–92. doi: 10.1016/j.molonc.2013.09.004

Figure 6.

Figure 6

ATM knockdown induced resistance to oxaliplatin in chemo‐naïve CRC cells. A. Western blotting showed a stable knockdown of ATM expression in HT29 cells. B. A lentiviral small interfering RNA‐induced knockdown of ATM led to increased resistance to oxaliplatin, as shown by a significantly right‐shift of the growth inhibition curve of oxaliplatin in HT29 cells 72 h post treatment (MTT assay). C. Knockdown of ATM (Sh‐ATM) induced resistance to oxaliplatin (Oxali), as shown by decreased levels of cleaved PARP and caspase‐3 (Casp3) at 48 h post treatment with 20 μM oxaliplatin. D. ATM knockdown cells (HT29‐ATMsh‐GFP) had significantly lower percentage of cell death than control cells (HT29‐GFP) at 48 h post treatment with 20 μM oxaliplatin. Quantification of cell death was by trypan blue staining. *P < 0.05. Results are representative of three experiments.