Table 1.
Primers for VEGF and VEGFR2 SNPs
| SNPa | Location | rs Number | Forward Primer | Reverse Primer |
|---|---|---|---|---|
| VEGF | ||||
| −2578 C/Ac | Promoter | 699947 | 5′-CTG CAT TCC CAT TCT CAG TC-3′ | 5′-TGC CCC AGG GAA CAA AGT TG-3′ |
| −2459ins/del18b | Promoter | 35569394 | 5′-CTG CAT TCC CAT TCT CAG TC-3′ | 5′-TGC CCC AGG GAA CAA AGT TG-3′ |
| −1498 C/Tc | Promoter | 833061 | 5′-TCT TCG AGA GTG AGG ACG TG-3′ | 5′-GGG ACA CAC AGA TCT ATT GG-3′ |
| −1154 G/Ac | Promoter | 1570360 | 5′-CGG GCC AGG CTT CAC TGA GC-3′ | 5′-CTC CCC GCT ACC AGC CGA CT-3′ |
| −634 G/Cc | 5′UTR | 2010963 | 5′-GCT CCA GAG AGA AGT CGA GG-3′ | 5′-GGT GTC TGT CTG TCT GTC CG-3′ |
| 936 C/Tc | 3′UTR | 3025039 | 5′-ACT CTG CGC AGA GCA CTT TG-3′ | 5′-TTC CGG GCT CGG TGA TTT AG-3′ |
| VEGFR | ||||
| 889 G/Ac | Exon 7 | 2305948 | 5′-TCT TGG TCA TCA GCC CAC TG-3′ | 5′-AAA CCC AGT CTG GGA GTG AG-3′ |
| 1416 A/Tc | Exon 11 | 1870377 | 5′-CTG GAA GTC CTC CAC ACT TC-3′ | 5′-TAC CAT GGT AGG CTG CGT TG-3′ |
Abbreviation: SNP, single nucleotide polymorphism.
a
Nomenclature as reported in Schneider et al.9
b
Identical primers were used for PCR and sequence analysis for SNP-2459 and −2578. These SNPs are 119 bp apart. The size of the PCR product that harbors these 2 SNPs is 143 bp.
c
The Hardy Weinberg P values for VEGF SNPs rs699947, rs833061, rs1570360, rs2010963, and rs3025039 were 0.06, 0.61, 0.10, 0.20, and 0.76, respectively; Hardy Weinberg P values for VEGFR2 SNPs rs2305948, and rs1870377 were 0.34 and 0.68, respectively.